It might just be that I never grew up in the original era, but I really prefer TNG-era.
IMO, best actors (Patrick Stewart ftw!), best ships (Enterprise E is a beast), and best movies (First Contact, Insurrection, and Nemesis).
It's not to say that the other Star Treks are bad, far far from it. I just don't find them as good as TNG. Voyager is a close second though. I kinda like DS9 too.
But Herc, if you want a good laugh, I have a video for you;
http://www.youtube.com/watch?v=55NwNrkzz4s